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In vitro fertilisation (or fertilization; IVF) is a process of fertilisation where an egg is combined with sperm outside the body, in vitro ("in glass"). The process involves monitoring and stimulating a woman's ovulatory process, removing an ovum or ova (egg or eggs) from the woman's ovaries and letting sperm fertilise them in a liquid in a laboratory. The fertilised egg (zygote) undergoes embryo culture for 2–6 days, and is then transferred to the same or another woman's uterus, with the intention of establishing a successful pregnancy. IVF is a type of assisted reproductive technology used for infertility treatment and gestational surrogacy, in which a fertilized egg is implanted into a surrogate's uterus, and the resulting child is genetically unrelated to the surrogate.
PrimedeQ has wide range of IVF Equipment, Air Pressurizing and Purification System, Anti Vibration Table, CO2 Incubator, CO2 Inline Filter, CO2 Regulator, Craft Oocyte Aspiration Pump, Cryogenic container, ICSI Systems, IVF Workstation, Makler Counting Chamber, Return Air Blower System, Stereo Zoom Microscope, Test Tube Warmer.
Intra Cytoplasmic Sperm Injection (ICSI) starts with a transvaginal oocyte retrieval procedure to extract one to several oocytes from a woman. Once the oocyte is retrieved, ICSI procedure is done under a microscope using multiple micromanipulation devices (micromanipulator, microinjectors and micropipettes). A holding pipette stabilizes the mature oocyte with gentle suction applied by a microinjector. From the opposite side a thin, hollow glass micropipette is used to collect a single sperm, having immobilised it by cutting its tail with the point of the micropipette. The oocyte is pierced through the oolemma and the sperm is directed into the inner part of the oocyte (cytoplasm). The sperm is then released into the oocyte. The oocyte polar body should be positioned at the 12 or 6 o'clock position, to ensure that the inserted micropipette does not disrupt the spindle inside the egg. After the procedure, the oocyte will be placed into an incubator for fertilization.
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